Figure 2. Effects of anakinra on viability of MDA-MB-231-IV tumours in bone.

Numbers of tumour cells undergoing apoptosis per mm² tumour (+/− SEM) were analysed following in situ hybridisation using a TUNEL kit a. and numbers of actively proliferating cells per mm² (+/− SEM) were assessed following Ki67 staining b. Photomicrographs show CD34 staining of endothelial cells in MDA-MB-231-IV tumours (T) in bone (B) 25 days after tumour cell injection c. numbers of CD34 positive blood vessels per mm² of tumour are shown in (c) and d. shows Endothelin 1 and VEGF gene expression in mouse bone and human MDA-MB-231-IV tumours growing in mouse bone following daily injection with anakinra compared with placebo. Panel f. shows numbers of F4/80+ve macrophages per mm² of tumour. * = p < 0.01 compared with placebo, ^ = P < 0.01 compared with placebo or treatment protocol.