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. 2016 Sep 28;7(46):75659–75671. doi: 10.18632/oncotarget.12318

Figure 3. Activation of lysosomal function by Curcumin is mTOR-dependent.

Figure 3

A. HCT116 cells were treated with Curcumin (10 μM) as indicated. B. Tsc2+/+ and Tsc2−/− MEFs were treated with Curcumin (20 μM) for 12 hours. Cells were harvested and lysed for western blotting to determine phospho-Akt (Ser473) and phospho-S6 (Ser235/236). β-actin was used as a loading control. C. as indicated in (B), LysoTracker Red staining was performed using confocal microscopy (Scale bar 10 μm) while MagicRed Cathepsin B D. and AO staining E. were also performed and were treated with Curcumin (20 μM) for 12 hours and analysed in Tsc2+/+ and Tsc2−/− MEFs using flow cytometry. All values are means ± SD at least 3 independent experiments and analysed using Student's t test (*P < 0.05).