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. 2016 Oct 4;7(46):75940–75953. doi: 10.18632/oncotarget.12445

Figure 1. Characterization of the peptide-Fc fusion proteins.

Figure 1

A. Schematic representation of WN-Fc-1 and WN-Fc-2 constructs. B. Analysis of purified Fc fusion proteins by SDS-PAGE followed by Coomassie staining. C. Western blot analysis with an anti-human Fc monoclonal antibody. Lanes 1 to 3 correspond to Fc control, WN-Fc-1, and WN-Fc-2 fusion proteins, respectively. D. Binding of Fc control, WN-Fc-1, and WN-Fc-2 to cancer cell lines and human CD4+ T cells. The cells were stained with the recombinant proteins (5μg/ml) and then analyzed by flow cytometry. Blue, orange and green histograms represent cells stained with Fc control, WN-Fc-1, or WN-Fc-2, respectively. Red histograms = cells stained with only FITC-conjugated anti-human Fc antibody. The data are from one single experiment and are representative of five independent experiments.