Figure 7. STAT3 and NF-κB signaling pathways are involved in IL-23-promoted self-renewal of CD133⁺ CSLCs.

A. Western blot analysis of p65 levels in cytoplasmic extract and nuclear extract from A2780-derived CD133⁺ CSLCs transfected with Control shRNA (vector) or IL-23p19-shRNA. B. Western blot analysis of p65 levels in cytoplasmic extract and nuclear extract from A2780-derived CD133⁺ CSLCs pretreated with DMSO or different concentrations of IL-23 inhibitor ST-5326 for 48h or untreated (Mock). C. Western blot analysis of p-STAT3 and STAT3 levels in total protein extracts from A2780-derived CD133⁺ CSLCs transfected with Control shRNA (vector) or IL-23p19-shRNA. D. Western blot analysis of p-STAT3 and STAT3 levels in total protein extract from A2780-derived CD133⁺ CSLCs pretreated with DMSO or different concentrations of IL-23 inhibitor STA-5326 for 48h or untreated (Mock).