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. 2016 Oct 12;7(46):76006–76020. doi: 10.18632/oncotarget.12579

Figure 8. STAT3 and NF-κB signaling pathways are involved in IL-23-promoted self-renewal of CD133+ CSLCs.

Figure 8

A. Immunofluorescence detection of p65 nuclear translocation in A2780-derived spheres transfected with Control shRNA (vector) or IL-23p19-shRNA and pretreated with DMSO or IL-23 inhibitor ST-5326 (10μM) for 48h. The upper right corner of the figures present the cell morphology with enlarged images. B. A2780-derived CD133+ cells transfected with Control shRNA (vector) or IL-23p19-shRNA were dissociated into 100 cells and stimulated with different concentrations of recombinant human IL-23, STAT3 signaling pathway inhibitor Cucurbitacin and NF-κB signaling pathway inhibitor PDTC. Sphere numbers were counted in each group. All experiments were performed three times and data are expressed as mean ± SD. *P<0.05, **P<0.01, ***P<0.001.