Figure 4. SOX17 antagonizes the canonical Wnt pathway in HEC-1B cells.

Cells were transfected with or without SOX17 cDNA together with the TCF-luciferase reporter (TOPflash) or mutant TCF-luciferase reporter (FOPflash) for 24 h. SOX17 knockdown repressed TOPflash activation in HEC-1B cells A. SOX17 overexpression increased TOPflash activation in HEC-1B cells B. SOX17/MAML3 co-transfection did not affect basal TOPflash activity C. HEC-1B and Ishikawa cells were transfected with SOX17 cDNA or shRNA alone or together with the MAML3 plasmid D. & E. Immunoblotting was performed on 10 μg of whole cell lysates. GAPDH was used as loading control.