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. 2016 Oct 15;7(46):76125–76139. doi: 10.18632/oncotarget.12682

Figure 3. Highly mesenchymal prostate cancer cells exhibit enhanced primary tumor incidence and size and in all cell lines CTC number is correlated with primary tumor size.

Figure 3

A. Incidence of primary tumor formation following orthotopic injection of PC-3M, PC-3, LNCaP C4-2B, and LNCaP human prostate cancer cell lines. Data are presented as the percentage of mice per cell line per timepoint with detectable primary tumors (n=6-39 mice/group) as assessed by microscopic histological examination of formalin-fixed, H&E stained tissue. * = significantly different (p<0.05), ** (p<0.01), *** (p<0.001). B. Mean combined weight of prostate and bladder at time of sacrifice following orthotopic injection of prostate cancer cell lines. Data are presented as the mean ± SEM (n=6-39 mice/group). C. Mean normalized number of CTCs/50μl of blood (assessed using the EMT semi-independent assay) correlates with the primary tumor weight in all cell lines. D. Representative H&E and IHC (E-cadherin and N-cadherin) images of primary prostate tumors for each cell line (40x; scale bars = 50 μm). Primary tumor weights were assessed for cell line variances using Levene's test, followed by a 1-way ANOVA with Tukey's post-test for multiple comparisons. Spearman rank correlation was utilized to examine the relationship between mean number of CTCs and primary tumor weight. * = significant difference relative to PC-3; α = significant difference relative to LNCaP C4-2B; δ = significant difference relative to LNCaP (p≤0.05).