Figure 1. Kindlin-1 expression is correlated with CRC progression and patient outcome.

Kindlin-1 expression in colorectal cancer patient specimens was examined by IHC. A. Kindlin-1expression level is higher in colon cancer tissue than in normal colon tissue. B. Kindlin-1expression levels in normal colon mucosa and tumor tissue from the same pair of patient were quantified (p=0.005). C. The expression level of Kindlin-1 in colorectal cancer tissues is correlated with AJCC grade. a. AJCC grade I. b. AJCC grade II. c. AJCC grade III. d. AJCC grade IV. D. High expression level of Kindlin-1 predicts a shorter overall survival for patients with colorectal cancer in Reid Colon Dataset of Oncomine (p=0.0399, Gehan-Breslow-Wilcoxon test). E. Kindlin-1 protein increases during CRC progression. a. The protein expression profile of Kindlin-1 was examined by Western blot analysis in eight colorectal cancer cell lines using an anti-Kindlin-1 antibody, controlled by actin loading. b. Relative protein band intensities were normalized with each β-actin band and were quantified. According to AJCC grade, the differences among groups I, II and III were analyzed by one-way ANOVA analysis and difference between groups are significantly. * indicates p<0.05 by Student's t test.