Figure 2. Kindlin-1 promotes CRC cell proliferation in vitro and tumor growth in vivo.

A. Kindlin-1 promotes CRC cell anchorage-independent growth. a. Kindlin-1 was overexpressed in SW1116 cells. b. Endogenous Kindlin-1 was depleted by specific small hairpin RNA in SW620 cells. c and d. Soft agar assays were performed using SW1116 with Kindlin-1 overexpression or SW620 with endogenous Kindlin-1 knockdown. Cells were mixed with soft agar and 3 weeks later colonies were photographed. Colonies larger than 50 μm in diameter were counted. Data were shown as mean ± SEM from three independent experiments. * indicates p<0.05 by Student's t test. B. Kindlin-1 promotes CRC cell proliferation. a and b. Cell growth was measured by WST-1 cell viability assay using SW1116 cells with Kindlin-1 overexpression. c and d. Cell growth was measured by WST-1 cell viability assay using SW620 cells with Kindlin-1 knockdown. Data were expressed as mean ± SEM. * indicates p<0.05 by Student's t test. C. Kindlin-1 is required for CRC tumor growth in mice. a. A representative nude mouse in tumor formation experiment. After six weeks, tumors were dissected. The upper panel of tumors was dissected from SW620 control shRNA stable cells, and the lower panel of tumors was dissected from SW620 Kindlin-1 shRNA stable cells. b and c. The average weight and volume of the tumors were quantified. Data were displayed as mean ± SD from a representative experiment. * indicates p<0.05 by Student's t-test.