Figure 7. Cytotoxic profile of MAIT cells.

A. MR1-independent activation. Freshly isolated PBMCs (1 × 10⁶/well) were incubated for 12 hours in the presence or absence of PMA (100 ng/ml) and IM (1 μM). B. MR1-dependent activation. Freshly isolated PBMCs (1 × 10⁶/well) were cocultured for 24 hours in the presence or absence of E. coli-infected THP-1 cells. Granzyme B, perforin and CD107a expressions in MAIT cells were determined by flow cytometry as described in the ‘Patients and Methods’ section. Data in (A and B) were obtained from 5 HCs. Values are expressed as the mean ± SEM. *, P < 0.001, **, P < 0.0001 by paired t test.