Fig. 3.

Inhibition of NF-kB p65 and STAT3 signal through transduction effect rhSOD3, but not rh209E, by Zn²⁺ in HaCaT. The cells were treated both TNF-α (10 ng/ml) and IFNγ (100 U/ml) and various concentration of ZnCl₂ (0–10 μM) (A). Purified rhSOD3 was added to cells at 200 U/ml (B). Amounts of p-NF-kB P65 in the cytosol was analyzed by western blot analysis. After purified rhSOD3 and rh209E were added to cells (with/without 200 U/mL), and added with/without ZnCl₂ (10 μM) (C). All proteins were analyzed by western blot analysis.