Table 1.

Assay details for real-time PCR of murine samples. UPL denotes Universal Probe Library fluorescent probe number (Roche Diagnostics Ltd, Burgess Hill, UK). Gene names: Acta2 = actin, alpha 2, smooth muscle; Anxa1 = annexin A1; Ccl4 = chemokine (C-C motif) ligand 4; Col1a1, Col1a2, Col3a1, Col4a1 = collagen, type 1-3-4, alpha 1-2; Cxcl = chemokine (C-X-C motif) ligand; Dusp1 = dual specificity phosphatase 1; E-selectin = selectin, endothelial; Gapdh = glyceraldehyde 3-phosphate dehydrogenase; Icam = intercellular adhesion molecule; Il = interleukin; Ifnγ = interferon gamma; L-selectin = selectin, lymphocytes; Mcp1 = monocyte chemoattractant protein 1; Mmp = matrix metallopeptidase; Pecam1 = platelet/endothelial cell adhesion molecule 1; P-selectin = selectin, platelets; Tbp = TATA-binding protein: Timp2 = tissue inhibitor of metalloproteinase 2; Tnf α = tumour necrosis factor alpha; VE-cadherin = vascular-endothelial cadherin; Vcam1 = vascular cell adhesion molecule 1; Vegfα = vascular endothelial growth factor alpha; Vegfr2 = vascular endothelial growth factor receptor 2.

Gene Symbol	Forward primer	Reverse Primer	UPL
Acta2	ctctcttccagccatctttcat	tataggtggtttcgtggatgc	58
Anxa1	ctttgccaagccatcctg	tgggatgtctagtttccacca	21
Ccl4	ccagcagtctttgctccaa	gctcactggggttagcaca	34
Col1a1	acctaagggtaccgctgga	tccagcttctccatctttgc	19
Col1a2	cacctggtcctgttggaagt	caccagggaagccagtca	9
Col3a1	tcccctggaatctgtgaatc	tgagtcgaattggggagaat	49
Col4a1	agttggaggaatgggcttg	ccagggacaccctgtgag	80
Cxcl1	gactccagccacactccaac	tgacagcgcagctcattg	83
Cxcl2	ccctggttcagaaaatcatcc	cttccgttgagggacagc	63
Dusp1	tggttcaacgaggctattgac	ggcaatgaacaaacactctcc	89
E-selectin	acagcagggcaacatgaaat	caactggacccattttggaa	48
FilaminA	tccctcagtcctttcaggtg	gcactttgacctgcagtgg	78
Gapdh	aggcaaaagacaccgtcaag	agaagatgcggctgtctctg	52
Icam1	ttggagctagcggaccag	ccggagctgaaaagttgtaga	80
Icam2	gcactcggagagtctcaaca	gctgcagcttcagtgtgact	75
Il1β	tgtaatgaaagacggcacacc	tcttctttgggtattgcttgg	78
Il6	gctaccaaactggatataatcagga	ccaggtagctatggtactccagaa	6
Ifnγ	ctcaggaagcggaaaagga	aaaattcaaatagtgctggcaga	60
Keratin6	gccaaggcagacagtctaaca	caggctacggttgttgtcc	55
L-selectin	tggtcatctccagagccaat	gcagtccatggtacccaact	47
Mcp1	catccacgtgttggctca	gatcatcttgctggtgaatgagt	62
Mmp2	tgcagggtggtggtcatag	tcacgctcttgagactttgg	78
Mmp9	cagaggtaacccacgtcagc	gggatccaccttctgagactt	7
Mmp10	gagtctggctcatgcctacc	caggaataagttggtccctga	81
Pecam1	cggtgttcagcgagatcc	actcgacaggatggaaatcac	45
P-selectin	agcgttgcaatgtccagagt	ggatccgagcagttcacct	48
Tbp	gggagaatcatggaccagaa	gatgggaattccaggagtca	97
Timp2	aggtaccagatgggctgtga	gtccatccagaggcactcat	52
Tnfα	ttgagatccatgccgttg	ctgtagcccacgtcgtagc	25
VE-cadherin	tcaccttctgtgaggagatgg	gatgatcagcaaggtaatcactgt	6
Vcam1	tcttacctgtgcgctgtgac	gacctccacctgggttctct	47
Vegfα	aaaaacgaaagcgcaagaaa	tttctccgctctgaacaagg	1
Vegfr2	accagagaccctcgttttca	catttgcttgcaggaggttt	22