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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Anal Bioanal Chem. 2016 Sep 30;409(2):579–588. doi: 10.1007/s00216-016-9934-9

Figure 2.

Figure 2

Global analysis of glycoproteins and glycopeptides identified by IsoTaG analysis of conditioned media from metabolically labeled PC-3 cells. A. Anti-biotin Western blot analysis of tagging and enrichment efficiency with DMSO control, Ac4ManNAl, and Ac4ManNAz samples. Total protein levels shown alongside (ponceau). Lanes: (1) protein input following CuAAC reaction with probe 1 or 2, (2) protein supernatant following overnight incubation with streptavidin–agarose beads, (3) aliquot of streptavidin–agarose beads (10 µL) after enrichment. B. Venn diagram of glycoproteins identified from nonconjugated on-bead tryptic peptides from Ac4ManNAz and Ac4ManNAl samples. C. Subcellular localization of proteins identified by direct intact glycopeptide assignments.