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. Author manuscript; available in PMC: 2018 Mar 7.
Published in final edited form as: Cell Metab. 2017 Feb 2;25(3):661–672. doi: 10.1016/j.cmet.2017.01.001

Figure 7. Cells ablated for PTH1R preferentially developed into adipocytes in vitro.

Figure 7

(A) Immunofluorescence staining of BMSCs cultured under adipogenic conditions derived from 3wk old Prx1Cre;Tmfl/+ and Prx1Cre;PTH1Rfl/fl,Tmfl/+ mice. Red: Prx1-Tomato positive cells, green: adipocytes expressing adiponectin, blue: DAPI stain for cell nuclei and yellow: double positive (red/green) cells rendering BMSCs undergoing adipogenic differentiation. Scale bar: 100μm. (B) The percentage of double positive cells in Prx1Cre;PTH1Rfl/fl,Tmfl/+ mice was significantly higher than in controls. n=3. **p<0.01 versus control. All graphs show mean ± SEM.