Figure 3.
THP-1 cellular uptake of nanoparticles. (A) Confocal images of MTP-BPLP-PLA-PLX4032 nanoparticles taken up and internalized by THP-1 cells. Nuclei were stained by DAPI; nanoparticles were shown in the FITC and PE-Texas Red channels, CD11b was immunostained by Alexa647 (pseudo color in pink). (B) FACS analysis of THP-1 cells before and after treated with BPLP-PLA-PLX4032 and MTP-BPLP-PLA-PLX4032 nanoparticles (C) FITC and PE-Texas Red average fluorescence intensity of THP-1 cells, THP-1 cells with BPLP-PLA-PLX4032 and MTP-BPLP-PLA-PLX4032 nanoparticles. *, p<0.01 compare to the THP-1 control. (D) THP-1 cell binding efficiency as the percentage of THP-1 cells that were labeled by BPLP-PLA-PLX4032 and MTP-BPLP-PLA-PLX4032 nanoparticles. *, p<0.01.