Fig. 5.

Blood and tissue samples from uninfected, CS, CR and CR/CT infected mice were diluted 1:5 in PBS and assayed by LD/MS using the Applied Biosystems Voyager DE-STR instrument. For each mouse (4 mice per group) 5 LDMS measurements were conducted. Each measurement consisted of twenty laser shot spectra. These 20 shot spectra were averaged and the averaged mVolt heme specific amplitudes were graphed. LDMS indicates that quantitatively more hemozoin was present in CS infected mice as compared to CR infected mice. There was a further dramatic reduction in the amount of hemozoin detected by LDMS in CR/CT mice as compared to CR drug untreated mice. In all groups the highest hemozoin levels were identified in liver followed by spleen with the lowest amount of hemozoin being present in blood.