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Tyrosine hydroxylase (TH) immunostaining in substantia nigra (SN) in the rat 6-OHDA lesion model. (A,B,D) Analysis of differences in TH positive cell counts in SN of the intact and lesioned side. ***p < 0.001 (t-test, N = 4/group). Scale bar is 100 μm. Error bars represent SEM. (C) Integral field-vision of the SN with TH immunostaining. The boxes indicate the SN in which positive neurons are counted (an area of 0.2 mm2).
