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. 2017 Mar 9;11:112. doi: 10.3389/fnins.2017.00112

Figure 2.

Figure 2

Experimental design and behavior changes induced by different treatment. (A) Timeline of experiments Studies began with cannula placement into the lateral ventricle and 6-OHDA lesions of MFB followed by apomorphine test identifying rats with complete lesions. The bottom arrow shows the timeline for all groups of chronic treatment with L-DOPA methyl ester (12 mg/Kg) plus benserazide (6 mg/Kg) once daily. Behavioral assessment was done every other day. The upper arrow shows the daily lateral ventricle infusion with vehicle or PD98059 half hour before L-DOPA injection. All animals were sacrificed on day 21 for further studies (N = 14/group). (B–E) Effect of PD98059 on AIMs in 6-OHDA-lesioned rats (B). ALO (total of axial, limb, and orofacial) AIMs scores that were obtained every 35 min over 140 min following the L-DOPA injection every other day. (C,D) Total ALO AIMs scores within 140 min sessions following drug administration on day 10 (C) and 20 (D). (E) Contralateral turns that were counted on days 1, 4, 8, 12, 16, and 20. (F,G) Stepping tests scores 15 min before (pre-) and after (post-) L-DOPA administration on day 2, 10, and 18. *p < 0.05, **p < 0.01 in (B–E), LID + PD98059 vs. LID + Vehicle, *p < 0.05 in (F,G), post-L-DOPA vs. pre-L-DOPA administration (one-way ANOVAs followed by Tukey HSD and LSD post-hoc test). Error bars represent SEM.