Fig. 1. Pharmacological characterization of hSlo3 currents.

(A) Representative traces of endogenous currents in HEK293 cells (upper panel; MOCK), currents in cells transfected only with the hLRRC52 subunit (middle panel; hLRRC52), and currents in cells co-transfected with hSlo3 and the hLRRC52 subunit (lower panel; hSlo3+hLRRC52). Currents were recorded using a step-depolarizing pulse from –100 to +140 mV, with a holding potential of –100 mV. (B) Upper panel: Pharmacological experiments designed to verify hSlo3 K⁺ currents, recorded as in (A) in the presence of TEA (20 mM), IbTx (100 nM), NH₄Cl (10 mM), or 4-AP (25 mM). Lower panel: Inhibition was calculated as the percentage of current remaining after treatment relative to that prior to treatment at 116 mV. (C) G~V curves shifted towards the left with increasing alkalinization and internal Ca²⁺. Left panel: hSlo3 G~V curves, tested at various pHi values: pH 6.0 (●), pH 7.3 (○), pH 8.0 (□), pH 9.0 (□). Right panel: hSlo3 G~V curves, tested at various [Ca²⁺]_i: DVF (●), 80 µM Ca (▲), 200 µM Ca (■), 1000 µM Ca (▼). Data are presented as averages of results from five patches. Lines are fits to the Boltzmann equation. V_h values for each Boltzmann component are indicated for each condition. The number of cells is indicated in a bar graph. ^*p<0.05.