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. 2017 Feb 21;21(2):267–273. doi: 10.4196/kjpp.2017.21.2.267

Fig. 2. PIG3 overexpression enhances the tumorigenicity of HCT116 cells in vitro.

Fig. 2

(A) Western blot analysis showed the increase of PIG3 expression in PIG3 overexpressing HCT 116 cells, compared to control vector expressing cells. a-tubulin was used as a loading control. (B) Control vector and PIG3 overexpressing cells were assessed for colony formation in soft agar for 14 days. (C) Cell migration and invasion assays were performed using the Transwell system. Data represent the mean cell numbers from 5 fields, ***p<0.001, compared to control.