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(A) Cells stably expressing control shRNA and PIG3-specific shRNA were analyzed by Western blot using an anti-PIG3 antibody. a-tubulin was used as a loading control. (B) Colony formation assay performed on soft agar. (C) Cell migration and invasion assays were performed using the Transwell system. Data represent the average cell numbers from 5 fields. ***p<0.001, compared to control.
