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. 2017 Mar 9;8:337. doi: 10.3389/fmicb.2017.00337

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Copyright © 2017 Wang, Zhi, Qiu, Shi and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intermediate accumulated by TYΔndpA_L and biotransformation of nicotine by Sphingomonas-ndpA, KT-ndpA and KT-ndpA_plus. (A) Nicotine with a maximum absorbance at 259 nm by UV scanning was accumulated by TYΔndpA_L; (B–D) biotransformation of nicotine by Sphingomonas-ndpA, KT-ndpA, and KT-ndpA_plus, shows that product was only formed in Sphingomonas-ndpA (product should be 6-hydroxynicotine with a maximum absorbance at 232 and 295 nm); (E) LC-MS analysis of the 6HN produced in (B).