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. 2017 Mar 9;8:337. doi: 10.3389/fmicb.2017.00337

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Copyright © 2017 Wang, Zhi, Qiu, Shi and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intermediate accumulated by TYΔndpB and biotransformation of 6HN by Sphingomonas-ndpB and KT-ndpB. (A) 6HN with a maximum absorbance at 232 and 295 nm by UV scanning was accumulated by TYΔndpB; (B,C) biotransformation of 6HN by Sphingomonas-ndpB and KT-ndpB, respectively, showed that no product was formed in strain KT-ndpB, whereas Sphingomonas-ndpB exhibited catalytic activity based on 6-hydroxy-N-methylmyosmine formation. LC-MS analysis of the product shown in (D), and 6HMM formed by purified NdpB demonstrates the same peak as in (D).