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Principal component analysis of the codon usage of nicotine-catalyzing genes in strains TY, SJY1, and S33. Black dots, genes from strain TY; red squares, genes from strain S33; green triangles, genes from strain SJY1. Most of the homologous genes in SJY1 and S33 were overlapping, and the genes in black circles were homologous genes in strains SJY1 and S33. ndpE and ndpF almost overlapped with each other in strain TY.
