Table 2.
Identification of markers from C. dahurica, C. foetida and C. heracleifolia.
| Compd. | tR (min) | Extractionmass | Mass | Formula | Characteristicfragment ion | Error(ppm) | Name | t-value | P-value | From |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 7.40 | 461.1089 | 462.1162 | C22H22O11 | 461[C22H21O11]− | −3.1 | 2-Feruloyl fukinolic acid-1-metyl ester | 6.74 | 3.12e−7 | C. foetida |
| 253[C11H9O7]− | ||||||||||
| 181[C9H9O4]− | ||||||||||
| 191[C10H7O4]− | ||||||||||
| 2 | 6.60 | 431.0984 | 432.1056 | C21H20O10 | 431[C21H19O10]− | −3.1 | 2-Feruloyl piscidic acid | 7.64 | 3.19e−8 | C. foetida |
| 193[C10H9O4]− | ||||||||||
| 149[C9H9O2]− | ||||||||||
| 3 | 13.03 | 665.3906 | 666.3979 | C37H58O11 | 677[C37H57O11]− | 3.9 | 7,8-Didehydro cimigenol-24-O-cimicifuga alcohol-3-O-β-D-xyl | −7.66 | 3.08e−8 | C. foetida |
| 617[C35H53O9]− | ||||||||||
| 4 | 14.90 | 634.3717 | 633.3644 | C35H54O10 | 633[C35H53O10]− | 3.2 | Cimicifugoside H-2 | −9.54 | 3.86e−10 | C. foetida |
| 5 | 16.70 | 679.4063 | 680.4136 | C37H60O11 | 679[C37H59O11]− | −0.8 | 12β-Hydroxy cohosh alcohol-3-O-β-D-gal | 6.06 | 1.79e−6 | C. heracleifolia |
| 619[C35H55O9]− | ||||||||||
| 6 | 16.26 | 677.3906 | 678.3979 | C37H58O11 | 677[C37H57O11]− | 0.5 | 7,8-Deoxy cohosh alcohol-24-O-acetyl alcohol-ara | 11.10 | 1.44e−11 | C. heracleifolia |
| 617[C35H53O9]− | ||||||||||
| 7 | 16.50 | 659.3801 | 660.3873 | C37H56O10 | 659[C37H55O10]− | −0.9 | 27-Deoxy Arcot hormone | 15.89 | 3.18e-15 | C. dahurica |
| 617[C35H53O9]− | ||||||||||
| 559[C32H47O8]− | ||||||||||
| 8 | 4.38 | 417.0827 | 418.0900 | C20H18O10 | 417[C20H17O10]− | −1.8 | Acimicifugic acid D | −5.85 | 3.14e−6 | C. dahurica |
Note: Compounds 1−4 were markers of C. foetida; Compounds 5−6 were markers of C. heracleifolia; Compounds 7−8 were markers of C. dahurica.