Table 1.

Characterization of HAEIBS by RP-HPLC.

Compound	Linear range (μg/mL)	t R (min)	Equationa	Linearity (R2)	Content (mg/g, mean ± SD)
Lawsone	3.25–52.00	6.3~6.7	Y = 33.38X − 62.247	0.9995	19.32 ± 1.03
Quercetin	7.00–56.00	9.3~9.7	Y = 44.70X − 271.25	0.9994	2.87 ± 0.82
MNQ	3.625–58.00	11.1~11.6	Y = 94.367X − 13.345	0.9996	31.15 ± 1.26
Kaempferol	3.625–58.00	15.2~15.7	Y = 56.855X + 53.611	0.9995	64.98 ± 1.18

^a y = ax + b, where y is the area of the peak and x is the concentration of the analyzed material. n = 3 replicates per day with a total n = 9 replicates over three separate days for each concentration.