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. 2017 Jan 19;150(4):432–443. doi: 10.1111/imm.12694

Figure 8.

Figure 8

Thioglycollate‐elicited eightless macrophages show intact endoplasmic reticulum (ER) stress response. (a–d) RT‐PCR analysis for ERDJ4 (a), ATF3 (b), ATF4 (c) and CHOP (D) mRNA relative expression normalized to the UBC housekeeping gene from treated (12–24 hr) and untreated wild‐type (grey bar) or eightless (black bar) peritoneal macrophages (n = 3). Tunicamycin‐treated wild‐type peritoneal macrophages served as a positive control for ER stress (white bar). (e) Representative results of unspliced and spliced XbpI in wild‐type (lanes 1 and 3) or eightless (lanes 2 and 4) macrophages untreated or treated with lipopolysaccharide (12 hr). T‐Tunicamycin (4 hr) treated wild‐type peritoneal macrophages. M‐DNA marker.