Figure 10.

Involvement of the nuclear factor (NF)‐κB signalling in detoxified listeriolysin O (dtLLO) proinflammatory activity. Human peripheral blood mononuclear cells (PBMCs) were treated with dtLLO in the presence or absence of fisetin at 7 and 28 μM concentrations. After 24 h, the supernatants were harvested and the production of (a) tumour necrosis factor (TNF)‐α, (b) interleukin (IL)‐6, (c) IL‐12 and (d) IL‐10 were measured by enzyme‐linked immunosorbent assay (ELISA). Fisetin abrogates the production of all the cytokines evaluated in a dose‐dependent manner. Significant P‐values for all comparisons are shown as follows: **P < 0·01; ***P < 0·001. Non‐significant P‐values are shown as n.s. One representative experiment of three independent experiments is shown.