Figure 1.

Peroxisome proliferator‐activated receptor‐β/δ (PPAR β/δ) promotes maturation and differentiation of bone‐marrow‐derived mast cells (BMMCs). BMMCs were isolated from adult female Pparβ/δ ^+/+ and Pparβ/δ ^−/− mice, and cultured in media containing interleukin‐3 (IL‐3) for 4 weeks followed by co‐stimulation with IL‐3 and stem cell factor (SCF) for 2 weeks. (a) Representative flow cytometric analyses of BMMCs from Pparβ/δ ^+/+ and Pparβ/δ ^−/− mice showing changes in expression of Fcε RI and c‐KIT from day 0 (D0) to day 39 (D39) of culture. The red dashed lines surrounding the two boxes illustrate a closer view of flow cytometric data on day 24 (D24). (b) After culturing for 24 days, the percentages of mature mast cells (MC), immature mast cells, basophils (BASO) and other cells (others) in total cell suspension were determined by flow cytometry. The relative mean fluorescent intensities (MFI) of (c) Fcε RI and (d) c‐KIT of BMMCs over time were determined by flow cytometry. Values represent the mean ± SEM. *Significantly different than Pparβ/δ ^+/+ mice, P ≤ 0·05. [Colour figure can be viewed at wileyonlinelibrary.com]