Figure 5.

Peroxisome proliferator‐activated receptor‐β/δ (PPAR β/δ) regulates mast cell mediators associated with activation of phospholipase Cγ1 (PLC γ1)and extracellular signal regulated kinase (ERK). (a) mRNA expression in resting bone‐marrow‐derived mast cells (BMMCs) of Cpa3, Mcp4, Cma1, Tspb2, Hexa and Hexb were determined by quantitative PCR. (b) The average concentrations of indicated inflammatory cytokines released from resting BMMCs were determined. (c) Quantitative Western blot analysis of pPLC γ1, PLC γ1, pPLC γ2, PLC γ2, pAKT, AKT, pp65, p65, pI κB, IκB, pp38, p38, pERK and ERK expression in resting BMMCs. Relative expression level of target protein was normalized to ACTIN. Values represent the mean ± SEM. *Significantly different than Pparβ/δ ^+/+, P ≤ 0·05. [Colour figure can be viewed at wileyonlinelibrary. com]