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. 2017 Feb 15;150(4):506–517. doi: 10.1111/imm.12711

Figure 3.

Figure 3

Western immunoblots probing extracts of peanut and mouse tissues with anti‐Schistosoma mansoni soluble egg antigen (anti‐SmSEA) IgG antibodies eluted by low pH buffer from a ~30 000–33 000 MW pair of peanut antigens. (M) Molecular weight marker. (a) Peanut, (b) normal mouse serum (NMS), (c) kidney, (d) spleen. Each strip was probed with: (1) eluted anti‐SmSEA IgG antibodies, (2) incubated only in 1 ml acid‐elution buffer, (3) rabbit anti‐NMS. [Colour figure can be viewed at wileyonlinelibrary.com]