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. 2017 Feb 15;150(4):506–517. doi: 10.1111/imm.12711

Figure 4.

Figure 4

Western immunoblot probing Schistosoma mansoni soluble egg antigens (SmSEA) and two purified egg antigens with eluted anti‐SmSEA IgG antibody to identify cross‐reactive egg antigens. (M) Molecular weight markers. (a) SmSEA probed with; (1) eluted anti‐SmSEA IgG antibodies, (2) rabbit anti‐IPSE/α‐1, (3) rabbit anti‐κ‐5, (4) incubated only in elution buffer, (5) rabbit anti‐SmSEA. (b) SDS–PAGE showing the purified egg antigens after staining in SimplyBlue SafeStain; (1) IPSE/α‐1, (2) κ‐5. (c) Purified IPSE/α‐1 probed with; (1) eluted anti‐SmSEA IgG antibodies, (2) rabbit anti‐IPSE/α‐1, (3) rabbit anti‐SmSEA, (4) incubated only in elution buffer. (d) purified κ‐5 probed with; (1) eluted anti‐SmSEA IgG antibodies, (2) rabbit anti‐κ‐5, (3) rabbit anti‐SmSEA, (4) incubated only in elution buffer. [Colour figure can be viewed at wileyonlinelibrary.com]