Figure 5.

Constituents of different plants probed with the acid‐eluted anti‐Schistosoma mansoni soluble egg antigen (anti‐SmSEA) IgG antibodies and the effect of treatment with periodate. (a) Incubated in 50 mm sodium acetate buffer before probing with eluted anti‐SmSEA IgG antibodies, (b) Incubated in 20 mm sodium metaperiodate dissolved in 50 mm sodium acetate buffer before probing with the same primary antibody as ‘a’. (M) Molecular weight marker, (1) unfractionated peanut, (2) purified pair of peanut antigens at ~30 000–33 000 MW, (3) crude latex (0·2 mg), (4) banana (0·16 mg), (5) tomato (0·27 mg), (6) melon (0·24 mg), (7) avocado (0·22 mg), (8) kiwi (0·19 mg), (9) SmSEA (0·010 mg). [Colour figure can be viewed at wileyonlinelibrary.com]