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. 2017 Mar 9;10:121. doi: 10.1186/s13104-017-2447-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — RTqPCR as a follow-up endpoint of RNA from human PCLS. a Efficiency calculation for B2M primer using several A549 cDNA dilutions. b Efficiency analysis for different RNA preparations from A549 cells and human PCLS (different donors). Results represent the means of at least three samples (RIN > 7.0). c MUC5AC expression in human PCLS as an example of lung-specific gene expression (calibration curve, melting curve, gel image)