Table 2.
RNA isolation from PCLS from different species using an optimized protocol
| Species | Incu-bation period | RNA yield (µg) | Absorbance 260/280 | RIN value | |||||
|---|---|---|---|---|---|---|---|---|---|
| Mean | Min. | Mean | Min. | p value | Mean | Min. | p value | ||
| Human (n = 12) | w/o | 1.12 ± 0.44 | 0.62 | 1.91 ± 0.07 | 1.79 | 8.9 ± 0.7 | 7.8 | ||
| Human, donor 1 (n = 12) | 3 days | 2.34 ± 1.03 | 1.34 | 1.90 ± 0.04 | 1.81 | 0.9107 | 8.7 ± 0.3 | 8.1 | 0.2691 |
| Human, donor 2 (n = 12) | 3 days | 1.52 ± 0.15 | 1.29 | 1.91 ± 0.09 | 1.75 | 0.4783 | 9.0 ± 0.4 | 8.6 | 0.7983 |
| Human, donor 3 (n = 12) | 3 days | 0.98 ± 0.28 | 0.56 | 1.88 ± 0.11 | 1.77 | 0.9701 | 9.0 ± 0.5 | 8.4 | 0.8112 |
| Rhesus (n = 5) | w/o | 2.02 ± 0.37 | 1.58 | 1.91 ± 0.06 | 1.87 | 8.2 ± 0.5 | 7.4 | ||
| Marmoset (n = 5) | w/o | 1.37 ± 0.16 | 1.16 | 1.87 ± 0.07 | 1.79 | 8.2 ± 0.3 | 7.8 | ||
| Rat (n = 5) | w/o | 3.80 ± 0.78 | 2.48 | 1.95 ± 0.04 | 1.91 | 8.3 ± 1.0 | 6.8 | ||
| Mouse (n = 5) | w/o | 5.67 ± 0.61 | 4.65 | 1.95 ± 0.01 | 1.93 | 8.2 ± 0.5 | 7.3 | ||
The average is indicated ± STABW. Student’s t-test was performed to compare absorbance 260/280 and RIN values with and without an incubation period, p-values are indicated
n indicates the number of analysed samples, w/o without