Table 3.

cRNA yield after sample preparation with 3′ IVT PLUS Reagent Kit (Affymetrix)

Material	Absorbance 260/230	Yield cRNA (µg)
A549 cells	1.90 ± 0.17	58.6 ± 15.6
Human PCLS	0.60 ± 0.21	51.5 ± 12.1

250 ng RNA were used as starting material. Results represent the means of 20 samples, all samples with A₂₆₀/A₂₈₀ ratios around 1.9 and RIN values above 8.0