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. 2017 Mar 9;10:137. doi: 10.1186/s13071-017-2067-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Identification of Eimeria species using species-specific primers amplifying the internal transcribed spacer 1 (ITS1) region. Seven pairs of primers specific for E. acervulina, E. brunetti, E. mitis, E. necatrix, E.maxima, E.praecox, and E.tenella were used to amplify the genomic DNA of field isolates, and the products were analyzed by agrose gel electrophoresis. One strain is shown as an example. Lane M: Trans2K Plus DNA Marker