Table 2. Wnt3a liposomes support serum-free derivation of human liver organoids.

Human liver tissue source	Underlying disease	Number of donors	Successful organoid derivations
Multi-organ donors	Cardiac arrest and/or brain death	4	3
End-stage liver disease patients	HCV combined with HCC	1	1
	α-1 Antitrypsin deficiency	1	1
	Wilson's disease	1	1

HCC, hepatocellular carcinoma; HCV, hepatitis C virus.

Derivations of organoids from biopsies of human liver obtained from multi-organ donors and end-stage liver disease patients in the presence of Wnt3a liposomes (800 ng Wnt3a per ml).