Figure 2. AN3661 efficacy in murine malaria models.

(a) AN3661 efficacy in P. berghei-infected mice. Mice (4–5 animals for each concentration tested) were treated orally with the indicated dosages of AN3661, chloroquine or vehicle daily for 4 days. Infections were monitored daily by Giemsa-stained blood smears, and mice were euthanized when parasitemias exceeded 50%. (b) AN3661 efficacy in P. falciparum-infected mice. Mice with ∼40% circulating human erythrocytes were intravenously infected with 2 × 10⁷ P. falciparum-infected erythrocytes on day 0, and AN3661 was then administered by oral gavage at the indicated dosages for 4 consecutive days (arrows). Parasitemia was measured by flow cytometry daily until day 7. Mean parasitemias for three mice in each group are shown. Error bars represent s.e.m.