Figure 7.

(a) SOD1 protein aggregates show significantly enhanced contrast in the amplitude reconstruction, imaged using the on-chip microscopy platform operating at 266 nm. (b),(d) and (e) The same aggregates imaged using a visible on-chip microscopy platform under blue (470 nm), green (532 nm) and red (633 nm) illumination light, respectively, all of which provide substantially lower contrast; also see Fig. 8. To provide a fair comparison we used the same number of frames, i.e., 556 frames, in panels a, b, d and e. The color bar represents the relative signal intensities in the holographic reconstructions. (c) and (f) SEM images of two representative protein aggregates of 483-nm (red arrow in (a)) and 443-nm (green arrow in (a)) in diameters.