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. 2017 Mar 9;7:44040. doi: 10.1038/srep44040

Figure 4. Scatterplot of the fluorescence emission values of thrombin FRET substrate solutions induced by the 23 compounds.

Figure 4

The fluorescence emission values of thrombin FRET substrate solutions were detected in the presence of 0.13 μg/ml thrombin incubated with the 23 compounds (30 μM in the total reaction system) using the SensoLyte™ 520 Thrombin Activity Assay kit in primary screening. Compounds 1 to 23 represent the 23 test compounds, i.e., salvianolic acid A, scutellarin, hyperin, piceid, L-epigallocatechin, L-arctigenin, 4-O-caffeoylquinic acid, 6-shogaol, oleuropein, chrysophanol, naringin, kaempferide, isoliensinine, salvianolic acid C, berberine, nobiletin, isoliquiritigenin, catalpol, neochlorogenic acid, byakangelicin, sesamolin, epicatechin and bilirubin. Compounds 24 and 25 represent the negative control (transporter solution) and positive control (600 nM argatroban), respectively. All error bars indicate the SE of three replicates.