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. 2017 Mar 6;8:14614. doi: 10.1038/ncomms14614

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Copyright © 2017, The Author(s)

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(a) By construction, the first principal component (PC1) of the EMPIRIC PCA is linearly related to average fitness (R=0.996, 'open circle' odd numbered libraries, 'filled circle' even numbered libraries). (b) A linear relationship between EMPIRIC PC1 and IGPS conservation of varying strength was observed for each orthologue. A linear correlation of R=0.408 was observed if all three orthologues were considered together. (c) The values of EMPIRIC PC1 vary with four-fold aligned positions, implicating structure in fitness determination. The canonical secondary structures are indicated above the plot. The green line indicates the average score. Highest scores were observed at both ends of the library positions, associated with the αβ and βα-loops (red boxes). Lowest average scores were observed in the intermediate positions, associated with the β-strands (blue boxes). (d) Average EMPIRIC PC1 scores based on the four-fold alignment correlate linearly with average four-fold IGPS conservation (R=0.842).