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. 2017 Mar 9;7:44332. doi: 10.1038/srep44332

Figure 4. cWFW triggers large-scale lipid domain formation in vivo.

Figure 4

(a) Phase contrast and fluorescence images of B. subtilis cells stained with fluorescent membrane dye nile red before addition (upper panels) and after 5 min (middle panels) and 20 min (lower panels) incubation with cWFW (12 μM). (b) Phase contrast and fluorescence images of B. subtilis cells stained with a 1:5 mix of NBD-labelled (FL-cWFW) and unlabelled cWFW (combined concentration of 12 μM) after 5 min (upper panels) and 20 min (lower panels) incubation. (c) Fluorescence images of B. subtilis cells stained with NBD-labelled peptide (FL-cWFW; left panel) and nile red (middle panel) upon 20 min incubation. The right panel depicts a colour overlay of the images shown in left and middle panels. (d) Phase contrast and fluorescence images of B. subtilis cells stained with fluorescent membrane dye nile red after 20 min incubation with cWFW (12 μM). Depicted are wild type cells (left panels), cells deficient for phosphatidylethanolamine (-PE, middle panels), and cells deficient for cardiolipin (-CL, right panels). (e) Fluorescence images of B. subtilis cell stained with laurdan upon 20 min incubation with cWFW (12 μM). Depicted is the laurdan emission at 450 nm (left panel), 520 nm (middle panel) and a colour-coded laurdan GP map calculated from the images shown in left and middle panels, respectively. Strains used: (a–e) B. subtilis 168 (wild type), (d) B. subtilis HB5343 (Δpsd, PE-deficient) and B. subtilis SDB206 (ΔclsA, ΔclsB, ΔywiE, CL-deficient).