Figure 2. The read length distribution and total transcripts identified in both the platforms and read coverage nature of the spermatozoal transcripts.

The read length distribution in (a) Ion Proton and (b) Illumina was analyzed by Bioanalyzer. The read length distribution differed significantly (p < 0.05) between platforms. (c) The total common genes identified in between the platforms were 10939 (TPM > 10) and 20898 (TPM > 1). The abundant transcripts were visually analyzed for reads coverage and observed that, the spermatozoal transcripts were exist in varying its nature/status. The spermatozoa contain (d) mature full length mRNA, (e) immature mRNA, (f) only exonic reads and (g) only intronic reads.