Figure 3. The spermatozoal RNAs retained specific regions/sequences for particular transcripts may influence fertility.

(a) The GPS2 transcripts had only exon retained region, where as (b) CHCHD2 had 3′ intronic retained region. Such RNA retained regions were consistently observed in all bulls and could be of fertility predictive value. (c) In order to get significant protein identities, genes (TPM > 1) obtained from the RNA-Seq were compared with the proteome data from LC-MS/MS (proteins with a minimum of 1 peptide per protein) and was observed that more than 61% of the proteins had corresponding transcripts in the bovine spermatozoa.