Table 3. The molecular functions and cellular localization of spermatozoal transcripts obtained from functional annotation analysis (Gene Ranker, Genomatix and DAVID bioinformatics) based on their transcription level (TPM).
| GO-Term | P-Value | Genes Observed |
|---|---|---|
| Molecular function | ||
| TPM > 100 | ||
| Structural constituent of ribosome | 1.04E-07 | 24 |
| Structural molecule activity | 1.88E-04 | 29 |
| Poly(A) RNA binding | 7.33E-03 | 29 |
| Ribonuclease A activity | 8.24E-03 | 2 |
| Endoribonuclease activity, producing 3′-Phosphomonoesters | 8.24E-03 | 2 |
| mRNA binding | 1.01E-02 | 6 |
| Copper ion binding | 1.14E-02 | 5 |
| Arp2/3 complex binding | 1.62E-02 | 2 |
| Photoreceptor activity | 1.62E-02 | 2 |
| TPM > 10 | ||
| Metal ion transmembrane transporter activity | 1.32E-04 | 55 |
| Ion transmembrane transporter activity | 4.48E-04 | 130 |
| Neurotransmitter:sodium symporter activity | 5.38E-04 | 9 |
| Substrate-specific transmembrane transporter activity | 5.73E-04 | 141 |
| Solute:sodium symporter activity | 7.06E-04 | 13 |
| Transmembrane transporter activity | 7.57E-04 | 148 |
| Structural molecule activity | 8.71E-04 | 141 |
| G-protein coupled amine receptor activity | 1.45E-03 | 10 |
| Structural constituent of ribosome | 1.48E-03 | 75 |
| Signaling receptor activity | 1.75E-03 | 103 |
| Cellular component | ||
| TPM > 100 | ||
| Cytosolic ribosome | 5.61E-09 | 19 |
| Ribosomal subunit | 3.66E-08 | 23 |
| Ribosome | 9.16E-08 | 27 |
| Cytosolic large ribosomal subunit | 1.61E-07 | 13 |
| Cytosolic part | 5.45E-07 | 21 |
| Large ribosomal subunit | 2.13E-05 | 13 |
| Ribonucleoprotein complex | 1.91E-04 | 33 |
| Intracellular non-membrane-bounded organelle | 4.38E-04 | 85 |
| Non-membrane-bounded organelle | 4.38E-04 | 85 |
| Small ribosomal subunit | 4.98E-04 | 10 |
| TPM > 10 | ||
| Cytosolic ribosome | 2.67E-06 | 52 |
| Cytosolic part | 2.85E-05 | 72 |
| Integral component of plasma membrane | 9.70E-05 | 102 |
| Intrinsic component of plasma membrane | 2.89E-04 | 105 |
| Cytosolic small ribosomal subunit | 3.00E-04 | 22 |
| Cytosolic large ribosomal subunit | 9.23E-04 | 29 |
| Ribosomal subunit | 1.01E-03 | 68 |
| Main axon | 1.12E-03 | 8 |
| Small ribosomal subunit | 1.40E-03 | 35 |
| Plasma membrane | 1.65E-03 | 450 |
The analysis was carried out using Bos taurus as background.