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. 2017 Mar 9;7:42895. doi: 10.1038/srep42895

Figure 2. Overexpression of Ascl1 in the dorsal telencephalon induces ectopically tangential migration.

Figure 2

Four days after electroporation to the dorsal telencephalon, brains of E19.5 rats were dissected and sectioned in the coronal plane. Electroporated cells were labeled with anti-GFP in green; nuclear DNA was stained with DAPI in blue. (A) A confocal image with a red square indicating the electroporated site. (B’–G’) are zoomed regions of (B–G) indicated by red squares. (B) In the control group. GFP-positive cells were distributed near the electroporated area and many of them extended processes radially. GFP-positive axons toward the contralateral side were observed (yellow dashed lines). (C) In Neurog2 group, most GFP-positive cells were distributed in the cortical plate (CP). GFP-positive axons toward the contralateral side were observed (yellow dashed lines). (D) In Ascl1 group, many GFP-positive cells were distributed in the VZ/SVZ (white arrows) and IZ (white arrowheads) dorsomedially to the electroporated site. (E) In Dlx2 group, many GFP-positive cells were distributed in the VZ/SVZ (white arrows) dorsomedially to the electroporated site. (F) In Ascl1 + shLacZ#1 group, GFP-positive cells were distributed in a similar pattern as Ascl1 group in (D). (G) In Ascl1+shDlx2#1 group, many GFP-positive cells were distributed in the IZ (white arrowheads) dorsomedially to the electroporated site. Few GFP-positive cells were distributed in the VZ/SVZ (white arrows). Length of the scale bar is 120 μm in (B–G), and 100 μm in (B’ to G’). (H) GFP-positive cells were categorized into radial, tangential, or other types according to the orientation of their leading processes. (I) Quantification of GFP-positive cells in the VZ/SVZ and IZ. Data are presented as box and whisker plots with all data points. The horizontal line within the box indicates the median, boundaries of the box indicate the 25th and 75th percentiles, and the whiskers indicate the maximum and minimum values of the results. Data are analyzed by using Student’s t-test, n = 6 in all groups. *p < 0.05; **p < 0.01.