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. 2017 Mar 9;7:42895. doi: 10.1038/srep42895

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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P19 cells were transfected with Neurog2 , Ascl1, or Dlx2 expression constructs and total RNA was extracted two days after transfection. The expression of Ephs (A,B) and Efns (C) were normalized to the expression of TATA-box binding protein (Tbp). Ephb1 and Ephb2 were expressed at higher levels in Ascl1-expressing cells than those in Neurog2 and Dlx2-expressing cells. Data are presented as box and whisker plots with all data points. Data are analyzed by using Student’s t-test, n = 4. *p < 0.05; **p < 0.01.