Figure 1.

Structure of the Integrin-Binding Region of Laminin-111

(A) Schematic drawing of laminin-111 mini-E8 (α1 chain, green; β1 chain, blue; γ1 chain, magenta). The disulfide bond between β1 and γ1 is indicated.

(B) Reducing and non-reducing SDS-PAGE analysis of mini-E8 pulled down from the 293F cell culture supernatant using Ni²⁺-affinity resin.

(C) Adhesion of HT1080 fibrosarcoma cells to mouse EHS tumor laminin-111 (Ln-111), recombinant E8, recombinant mini-E8 (mE8), and their respective γ1 E1605Q mutants (mut). Data shown are representative of four independent experiments.

(D) Two orthogonal views of the mini-E8 crystal structure. The disulfide bond linking the C termini of the β1 and γ1 chains is shown in orange. The Ca²⁺ ions in LG1 and LG2 are shown as salmon-colored spheres. The C-terminal residue of the γ1 chain in this structure is Glu1605. See also Figure S1.