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. 2017 Feb 9;18(2):77. doi: 10.3390/ijms18020077

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of GP-17 on ERα in Ox-LDL-treated HUVECs. (A) Localization of ERα immunoreactivity was identified in the cytoplasm by immunofluorescence assays; (B) Localization of ERα immunoreactivity was identified in the plasma membrane by immunofluorescence assay; (C,D) The expressions of both ERα and ERβ were analyzed by western blots of Ox-LDL-induced HUVECs. The values are expressed as the mean ± S.E.M. from three independent experiments. ^## p < 0.01 vs. Control; * p < 0.05 vs. Ox-LDL; ^& p < 0.05 vs. GP-17 + Ox-LD. DAPI, 4,6-diamidino-2-phenylindole.